Figure 4 | Scientific Reports

Figure 4

From: Hic-5 is required for activation of pancreatic stellate cells and development of pancreatic fibrosis in chronic pancreatitis

Figure 4

Hic-5 knockdown attenuates activation of cultured human PSCs by impairing Smad2 phosphorylation. (A) The expression of Hic-5 and α-SMA was examined by western blot analysis. N: control siRNA. S1 and S2: hic-5 siRNAs. (B) Comparison of Hic-5, α-SMA and COL1a1 relative mRNA expression in the control and hic-5 siRNA transfection group. (C) siRNA was transfected into human PSCs. The expression of Hic-5 (red) and α-SMA (green) was examined by immunofluorescence. Scale bars indicate 20 μm (D) Representative western blot analysis of p-Smad2, Smad2 and Hic-5 expression in cultured human PSCs after transfection with control siRNA or Hic-5 siRNA. (E) Quantitative analysis of p-Smad2. **p < 0.01, *p < 0.05.

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