Figure 5
From: Oxidative stress markers in patient-derived non-cancerous cervical tissues and cells
The levels of DNA damage approximately mirror the observed ROS levels in the cervical keratinocytes. DNA damage was assessed by detection of 8-oxodG using Avidin-FITC. Cells were fixed in 4% paraformaldehyde, permeabilized with ethanol, and stained with 2 µg/ml of Avidin-FITC for 1 h. The level of bound Avidin-FITC, presented as mean fluorescence intensity (MFI (Avidin-FITC)) was detected by flow cytometry and analyzed using FlowJo software. The MFI values for DNA damage (Avidin-FITC staining) were plotted as bars on a graph displaying the levels of ROS in an increasing order as a line plot. To determine whether the variances in ROS levels and in 8-oxodG levels were significantly different, the F-test was used.
