Figure 3
Analysis of cell phenotype for the hinge area of first-generation TEHVs. Immunofluorescence images at the hinge area stained for M1 (red) and M2 (green) macrophages at 8 (A), 16 (B), and 24 (C) weeks follow-up. (D) Total cell number quantification in the wall and hinge region of first-generation TEHVs is showing an abundant cell presence indicating a high ongoing inflammatory response. (E) Quantification of M1 and M2 macrophages in the hinge region at the different time-points considered shows a prevalence of M1 over M2 subpopulation, with the number of M1 macrophages gradually decreasing over time while M2 macrophages is increasing. For immunofluorescence pictures: scale bars: 100 μm, zoom 1 × . For every measurement, cell number is normalized per μm3.
