Figure 5
From: Pulmonary surfactant inhibition of nanoparticle uptake by alveolar epithelial cells
(a–d) Superimposed red fluorescence and phase contrast images of A549 exposed to silica nanoparticles at 100 µg ml−1 (a,c) and silica nanoparticles at 100 µg ml−1 mixed with Curosurf at 200 µg ml−1, X = 2 (b,d), on glass (a,b) and on transwell (c,d). Scale bar 50 µm (a,b) and 25 µm (c,d). (e,f) Quantitative analysis of integrated intensity of the fluorescence signal from the three exposure conditions, namely silica at 100 µg ml−1 and silica at 100 µg ml−1 mixed with Curosurf at 200 µg ml−1 (X = 2) and 2 mg ml−1 (X = 20). Each exposure condition was tested in duplicates.
