Figure 8

Confocal microscopy images of NCI-H441 exposure to silica nanoparticles at 100 µg ml⁻¹ (a,c) and silica at 100 µg ml⁻¹ mixed with Curosurf at 200 µg ml⁻¹, X = 2 (b,d) on glass (a,b) and on transwell (c,d). The x–y plane images are merges of phase contrast and red, green and blue confocal stacks. The colors are respectively from Cy3-tagged nanoparticles, PKH67-tagged Curosurf and DAPI-tagged nuclei. The presented y–z plane images are merges of red, green and blue confocal stacks, left is bottom and right is top. The triangle positions the y–z plane. Scale bar 10 µm (x–y), and 5 µm (y–z). (e,f) Semi-quantitative analysis of area fraction of nanoparticles per individual cells for three exposure conditions, namely neat silica nanoparticles, and nanoparticles mixed with Curosurf at X = 2 and X = 20.