Table 1 Tissue cryo-preservation timeline.
From: A novel cryo-embedding method for in-depth analysis of craniofacial mini pig bone specimens
Solution | Duration | Conditions |
|---|---|---|
4% PFA | 72 h | 4 °C |
PBS or 70% ethanol | As needed | 4 °C |
30% sucrose | 48 h | 4 °C, slow shaking |
30% sucrose | 48 h | 4 °C, slow shaking |
30% sucrose | 48 h | 4 °C, slow shaking |
30% sucrose | 48 h | 4 °C, slow shaking |
30% sucrose:PEO-CMC (1:1) | 48 h | 4 °C, slow shaking |
30% sucrose:PEO-CMC (1:2.5) | 48 h | 4 °C, slow shaking |
30% sucrose:PEO-CMC (1:4) | 48 h | 4 °C, slow shaking |
PEO-CMC | 48 h | 4 °C, slow shaking |
