Figure 1 | Scientific Reports

Figure 1

From: Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Figure 1

Flow cytometry scatter plots and gating method. (a) Analysis of size-specific beads shows five distinct clusters. (b) A gate is set to capture events from the 9 μm measurement and above. (c) Events detected in this region are replotted to determine SYB14 and PI absorbance. Events in the Q3 region are SYB14 positive and PI negative and therefore viable. In samples treated with a negative control (d), the majority of events falls in the Q1 region, with only propidium iodide detected (e).

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