Figure 3
From: Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis
Enrichment of Vasa-positive spermatogonial cells after sorting a cryopreserved testis cell sample. (a) A cell suspension is analysed by the flow cytometer and two gates were set, the “A” gate and the “B” gate. (b) Measurements of cell sizes in the unsorted sample, “A” gate and “B” gate showed the “A” gate captures a significantly higher proportion of large testis cells > 9 μm (Chi-square, p < 0.001). (c) Staining cell samples from each group showed the “A” gate had a higher percentage of Vasa-positive cells compared to an unsorted sample (un-paired T-test, p = 0.0251), no Vasa-positive cells were detected through the “B” gate thus this data was not included in analysis. (d) Images of cells from all three treatment groups, including live cells in suspension and fixed cells stained with Vasa and Hoechst. Cell sizes in stained images differ in comparison to live images due to the dessication of cells during fixation to slides, a figure to support this is inlcuded in the supplementary material.
