Figure 2 | Scientific Reports

Figure 2

From: Deviant reporter expression and P2X4 passenger gene overexpression in the soluble EGFP BAC transgenic P2X7 reporter mouse model

Figure 2

Comparison of EGFP, P2X7, and P2X4 transcript levels and expression patterns in sEGFP and P2X7-EGFP BAC transgenic P2X7 reporter mice. (A) In situ hybridization analysis of EGFP, P2X7, and P2X4 transcript levels in the two reporter mouse models in comparison to wt animals. Representative coronal sections (Bregma 0.62 approximately) are displayed. The signal intensities of 12 representative areas were measured on three different mice per group. The background noise was subtracted and the resulting values were normalized to values in wt animals. Bars represent mean ± SEM from three animals analysed in three independent experiments. (B) Region-specific quantitative PCR of P2X7 and P2X4 mRNA levels in the P2X7-EGFP and sEGFP mouse lines. RNA levels were normalized to levels in wt animals as well as a housekeeping gene (HPRT) which correspond to the dotted line. Bars represent mean ± SEM from three animals. (C) Immunofluorescence staining for EGFP in the P2X7-EGFP and sEGFP lines. Coronal sections from approximately Bregma 0.62 are shown.

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