Figure 3

Comparison of EGFP and P2X7 protein expression patterns in sagittal brain sections of sEGFP and P2X7-EGFP BAC transgenic P2X7 reporter mice. (A) Immunofluorescence staining with an anti-GFP antibody. Note that signal intensities were individually adjusted to prevent saturation in the sEGFP mouse. Scale bars 1 mm. (B) DAB staining with an anti-GFP antibody. (C) DAB staining using a P2X7-specific nanobody. Scale bars: 1 mm in whole brain, 200 µm in insets. Representative stainings from 3 different animals per group are shown.