Figure 3
Live cell imaging using Green Lindoblum and Green Pegassos. (a,b) Top, sequential pseudo-colour images of HEK293T cells expressing Green Lindoblum (a) or HeLa cells expressing Green Pegassos (b). Scale bar represents 30 µm. Bottom, time courses of fluorescence intensity (FI) in the cells during the application of 1 mM lactate (a) or 100 µM and 1 mM pyruvate (b). (c) Time courses of FI of Green Lindoblum (green line) and Rhod2 (red line) in HeLa cells during the application of 1 mM lactate and 100 µM histamine. (d) Time courses of FI of Green Pegassos (green line) and Pink Flamindo (red line) in human iPS cell-derived cardiomyocytes during the application of 30 mM glucose. The data are shown as means ± standard deviation (n = 85 cells from five independent experiments (a), 28 (b), 35 (c) and 23 (d) cells from three independent experiments). The RB without stimuli was perfused as shown in white bars.
