Figure 7

Gephyrin protein expression is reduced in the nAc of 2Tg mice. (a) Immunostaining of coronal slices (30 μm) and high magnification images (insets) showing gephyrin protein in the nAc from WT and 2xTg mice. (b) Quantification of gephyrin fluorescence intensity in the soma of nAc neurons (dashed circles in (a) show regions of interest). In the 2xTg mice, gephyrin fluorescence intensity was significantly decreased. Data are mean ± SEM and each data point reflects one soma (***p < 0.001). (c) Western blot of nAc from WT and 2xTg animals for gephyrin and Gβ (loading control). Western blot analysis shows lower levels of gephyrin in 2xTg compared with WT mice (Black lines at the edge of each blot indicates cropping from original full-length blots available in Supplementary Fig. 5). Data are mean ± SEM and each data point reflects one experiment (t(5.112) = 3.146, *p = 0.0247). Mann–Whitney test for (b) and Unpaired Student’s t-test with Welch’s correction for (c). n = 3 WT and 2xTg mice. Images were analyzed with ‘ImageJ’ 1.8.0_112 (NIH), https://imagej.nih.gov/ij.