Figure 8

Expression of GlyRα is reduced in the nAc of 2xTg mice. (a) Immunostaining of nAc coronal slices (30 μm) and high magnification images (insets) showing GlyRα protein in the nAc from WT and 2xTg mice. (b) Quantification of GlyRα fluorescence intensity in the soma of nAc neurons (dashed circles in (a) show regions of interest). GlyRα fluorescence intensity was significantly decreased in 2xTg mice. Data are mean ± SEM and each data point reflects one soma (p < 0.0001). (c) Western blot of nAc from WT and 2xTg animals for GlyRα and Gβ (loading control). Western blot analysis shows lower levels of GlyRα in 2xTg compared with WT mice (Black lines at the edge of each blot indicates cropping from original full-length blots available in Supplementary Fig. 5). Data are mean ± SEM and each data point reflects one experiment (t(9.688) = 3.546, p = 0.0056). Mann–Whitney test for (b), Unpaired Student’s t-test with Welch’s correction for (c). **p < 0.01, ***p < 0.001. n = 4 WT and 2xTg mice. Images were analyzed with ‘ImageJ’ 1.8.0_112 (NIH), https://imagej.nih.gov/ij.