Figure 4

Thermal stability at 20 °C and 40 °C after 3 and 24 h (a), storage stability at 4 °C after 21 days (b) of immobilized ADH-CMD-MNPs and soluble ADH, reusability of immobilized ADH-CMD-MNPs after 18 consecutive cycles (c) and Lineweaver–Burk double reciprocal plot for soluble ADH and immobilized ADH-CMD-MNPs; substrate ethanol concentration was varied between 0.3 and 0.8 M for both soluble and immobilized ADH-CMD-MNPs (d). (Standard deviation for all samples was less than 2%).