Table 2 Constant process conditions during the optimization process of each single-factor experiment.

From: Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Optimizing condition

Constant process conditions

EClH concentration [% (v/v)]

pH 7.5, 400 rpm, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time

Immobilization temperature (°C)

4% (v/v) EClH, pH 7.5, 400 rpm, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time

Rotation speed (rpm)

4% (v/v) EClH, pH 7.5, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time

pH of the medium (/)

4% (v/v) EClH, 500 rpm, 4 °C, enzyme concentration 0.02 mg/mL, 2 h immobilization time

Immobilization time (h)

4% (v/v) EClH, pH 7.5, 500 rpm, 4 °C, enzyme concentration 0.02 mg/mL

ADH concentration (mg/mL)

4% (v/v) EClH, pH 7.5, 500 rpm, 4 °C, 2 h immobilization time

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