Figure 3

Functional analysis of significantly differential TF motifs between the AS_PBMC and NC_PBMC libraries. (a) GO analysis of 12,430 target genes related to the 29 recorded significantly differential TF motifs; KEGG analysis revealing the 20 most significant pathways involved in (b) the 21 more accessible TF motifs and (c) the 8 less enriched ones from the AS_PBMC group; (d) Boxplot showing counts of the 8 less active TF motifs from dendritic cells, monocytes-1 and T cells; Enriched KEGG analysis displaying the 5 most significant pathways related to TF motifs (e) OLIG (OLIGI and OLIG2), (f) NR1H4, and (g) TEAD1 and JUN. *, p < 0.05; **, p < 0.01, ***p < 0.001, p-value was calculated with Loupe Cell Browser 3.1.1 through the difference analysis part, and it was adjusted using the Benjimini-Hochberg correction for multiple tests. TF, transcription factor; PBMCs, peripheral blood mononuclear cells; AS_PBMC, PBMCs from patients with ankylosing spondylitis (AS); NC_PBMC, PBMCs from healthy controls; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.