Figure 3
From: Integrating single-cell RNA-seq and imaging with SCOPE-seq2
Validation and performance of SCOPE-seq2 in a mixed-species experiment. Saturation analysis of the number of (A) unique transcript molecules and (B) genes detected per cell (violin plots indicate distributions across cells). Comparative analysis of SCOPE-seq and SCOPE-seq2 for the number of (C) unique transcript molecules and (D) genes detected per cell (violin plots indicate distributions across cells). Scatter plots showing the number of uniquely aligned human and mouse reads corresponding to each cell barcode linked to images, before (E) and after (F) removal of multiplets. Each point (cell) is colored by the fluorescence intensity ratio of the human and mouse live staining channels, indicating excellent agreement between scRNA-seq and imaging.
