Figure 4
Selection of residues, presumably located near a bicyclic or monocyclic structure, during the second step of the reaction. (a) Structures of substrates 6, 7, and 10. Positions corresponding to bicyclic or monocyclic structure on the substrates are shown in yellow. (b) Homology model of BmeTC. The 3D structure of BmeTC was modeled using SWISS-MODEL. The structure was constructed using the coordinates of SHC complexed with 2-azasqualene inhibitor [Protein Data Bank (PDB) code: 1UMP] as a template and displayed using PYMOL. Blue: 2-azasqualene; pink and green: residues within 4 Å of 2-azasqualene; pink: residues targeted in this study; yellow: positions corresponding to bicyclic or monocyclic structure on the substrates.
