Table 1 Characteristics and genetic diversity statistics of the 10 polymorphic microsatellite markers developed for Warburgia salutaris.
Locus | Repeat motif | Accession number | Primer Sequence 5′–3′ | Size range | Na | Ho | He |
|---|---|---|---|---|---|---|---|
1-N1002135 | (ATG)5 | MT515706 | F: TATGTTGGGAGAGGGTGAGG R: GTTTAACGACTGCATCATCCCA | 132–174 | 6 | 0.487 ± 0.139 | 0.394 ± 0.101 |
7-N1082598 | (AAT)9 | MT515707 | F: GTTGATCATAGACACGCCAAGG R: GTCGTGCAACCTAGAGGTCC | 161–182 | 7 | 0.633 ± 0.085 | 0.700 ± 0.029 |
10-N1110523 | (TTA)9 | MT515708 | F: AACCATTGGCACCTCAAGTC R: GTTGAAGTTGAGGGAAGGGATG | 244–262 | 7 | 0.852 ± 0.060 | 0.786 ± 0.023 |
12-N1126672 | (TTG)7 | MT515709 | F: GTTAAATCTGGACCCACTTGCC R: GGGTGAATTAGTGAACGTCTTG | 161–180 | 7 | 0.805 ± 0.125 | 0.718 ± 0.074 |
13-N1132836 | (AAG)7 | MT515710 | F: GTTCCTGCTCCGAGACCTAGAA R: TCATGAAGAAATCGCAACCA | 138–144 | 3 | 0.304 ± 0.087 | 0.296 ± 0.086 |
16-N1150626 | (TGG)5 | MT515711 | F. GTCTTTGGCGAAATCAGTTGGT R: GAAGGTTTCCAGGTTGGTGA | 149–159 | 3 | 0.299 ± 0.186 | 0.249 ± 0.109 |
18-N1173706 | (AAG)6 | MT515712 | F: GAGCTGCCTCGATATGGACT R: GTTATCCAATGGCCAAGAAACC | 164–170 | 3 | 0.398 ± 0.105 | 0.421 ± 0.078 |
31-N2284857 | (TTC)12 | MT515713 | F: GTCTCTTGCTATCATGCGGTCA R: CAGATTGGAGAATCCAGACCA | 207–263 | 9 | 0.771 ± 0.138 | 0.812 ± 0.078 |
33-N3477883 | (TGA)6 | MT515714 | F: GTACAAGATTCATGTGACCGGC R: GCAAGGCATCATATTCACGA | 184–200 | 4 | 0.550 ± 0.171 | 0.472 ± 0.124 |
43-N1009973 | (AT)10 | MT515715 | F: GTTGCGCTCATCGATCTGTA R: GTGCGAACTATGATCGGACGAA | 146–185 | 9 | 0.439 ± 0.102 | 0.778 ± 0.027 |
