Table 2 Composition of PCR master mix for COI and 16S rRNA.

From: Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus

Components

Volume/reaction (μL)

Template (200 ng/µL)

0.5

Buffer (10 ×)

1.25

Forward primer

0.5

Reverse primer

0.5

dNTPs (10 mM)

0.25

Taq polymerase (5 units/µL)

0.15

D/W

9.35

The total volume was 12.5 µL

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