Table 3 Thermal regime for cytochrome C oxidase I (COI) gene.

From: Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus

Steps

Condition

Cycles

Temperature (°C)

Time

Initial denaturation

94

5 min

1 cycle

Denaturation

94

30 s

35 cycles

Annealing

54

30 s

Extension

72

45 s

Final extension

72

7 min

1 cycle

Soak

4

Forever

–

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