Figure 7
Effect of GLA on neurite outgrowth. (A,B) Immunocytochemistry of primary cortical neurons at 1 and 7 DIV. Various GLA concentrations were added to the culture medium. Arrowhead indicated axonal degeneration. (C) Tuj1+ neurite length quantification at 1 DIV is shown. GLA reduced neurite outgrowth in a concentration-dependent manner. (D) Neuronal viability quantification at 1 and 7 DIV is shown. (E) A schematic diagram of abnormal cortical development of perinatal GLA exposure is depicted. Error bars represent the S.E.M; n.s., not significant; *p < 0.05 compared with controls; Kruskal–Wallis one-way analysis of variance on ranks with Dunn’s method. Scale bars: B, 100 μm; GLA glufosinate-ammonium; DIV days in vitro; S.E.M. standard error of the mean.
