Figure 1 | Scientific Reports

Figure 1

From: Inactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death

Figure 1

Med22 is expressed highly by podocytes. (A) qPCR analysis of human kidney tissue shows that Med22 is expressed more strongly by glomerular tissue (glom) than rest of kidney fraction (rok). Podocin, a podocyte-specific gene, was used to control purity of glomerular fractions. (B) Immunofluorescence staining for Med22 in adult human kidney shows strong signal in glomeruli (G) and only sparse reactivity outside glomeruli. (C) Double labelling experiments with vimentin (red) shows partial co-localization (yellow) in podocyte major processes. No significant overlapping reactivity is observed with foot process marker nephrin (red), mesangial marker alpha-smooth muscle actin (ASMA, red) or endothelial marker CD31 (red).

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