Figure 4 | Scientific Reports

Figure 4

From: Inactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death

Figure 4

Electron microscopy of pod-Med22 kidneys shows progressive vesicular lesions in podocytes. (A) At 8 weeks, pod-Med22 mice show podocyte foot process effacement as validated by calculating number of slits per GBM length. (B) At 12 weeks, vesicular structures (*) are regularly detected in podocyte (P) cytoplasm. Occasionally, the vesicles are obstructing podocyte nuclei. Vesicular membranes (VM) lack electron-dense surface that is present on plasma membrane (PM) of podocytes. (C) At 16 weeks of age, glomeruli show areas of GBM that are not covered by podocytes (arrowheads) indicating podocyte loss. Moreover, completely sclerotic glomeruli are detected. US urinary space; CL capillary lumen; fp foot process. *p < 0.05.

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