Table 1 Precursor and fragment ion transitions and their charge states monitored by PRM for HLA α-chain quantification.

From: Human leukocyte antigen class II quantification by targeted mass spectrometry in dendritic-like cell lines and monocyte-derived dendritic cells

HLA-α type

Surrogate tryptic peptide

Precursor to fragment Ions

DRA1/DQA2

VEHWGLDEPLLK

479.2575+++ (light)

479.2575+++ (heavy)

E [y5] − 599.3763 + 

E [y5] − 607.3905+ 

E [y11] − 668.8484++

E [y11] − 672.8555++

H [y10 ] − 604.3271++

H [y10] − 608.3342++

DPA1

VEHWGLDQPLLK

478.9295+++ (light)

481.6009+++ (heavy)

Q [y5] − 598.3923+ 

Q [y5] − 606.4065+ 

E [y11] − 668.3564++

E [y11] − 672.3635++

H [y10] − 603.8351++

[H [y10] − 607.8422++

W [y9] − 535.3057++

W [y9] − 539.3128++

DQA1

VEHWGLDKPLLK

478.9416+++ (light)

481.6130+++(heavy)

K [y5] − 598.4287 + 

K [y5] − 606.4429 + 

E [y11] − 668.3746++

E [y11] − 672.3817++

H [y10] − 603.8533++

H [y10] − 607.8604++

W [y9] − 535.3239++

W [y9] − 539.3310++

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