Figure 3
Effect of Eucalyptus oil (Eu) and 1,8-cineole on IgE-induced degranulation in bone-marrow-derived mast cells (BMMCs). (a) Effect of Eucalyptus oil and 1,8-cineole on BMMCs viability. Cells were treated with various concentrations of Eucalyptus oil or 1,8-cineole for 24 h. Next, cell viability was measured using the MTT assay. Relative cell viability was calculated by comparing the absorbance in cells treated with Eucalyptus oil or 1,8-cineole with that in cells treated with vehicle (0.1% DMSO). Data are expressed as the mean ± SEM (N = 3). (b) Eucalyptus oil or 1,8-cineole was applied to anti-DNP-IgE-sensitised BMMCs immediately after stimulation with DNP-human serum albumin (HSA), and after 1 h, β-hexosaminidase release was determined by comparing the enzymatic activity in BMMCs between treatment with vehicle (0.1% DMSO) and DNP-HSA. Data are expressed as the mean ± SEM (N = 4). Dunnett’s test was used to assess the statistical significance (**P < 0.01versus vehicle-DNP-HSA).
