Figure 5

1,8-cineole suppresses the elevation of intracellular Ca²⁺ concentration. BMMCs sensitised with anti-DNP IgE were incubated in medium containing Fluo 4-AM. BMMCs were treated with various concentrations of 1,8-cineole and stimulated with DNP-HSA 60 s after initiating the monitoring of fluorescence intensity. (a) Time course of intracellular Ca^{2 +} concentration in BMMCs. Data are displayed as mean ± SEM (N = 5). (b) Relative fluorescence intensity 60 s after DNP-HSA stimulation. Data are displayed as mean ± SEM (N = 5). Statistical significance was assessed using Dunnett’s test (** P < 0.01 vs vehicle-DNP-HSA).