Figure 6
Effect of 1,8-cineole on the phosphorylation of Syk and Lyn. (a,b) Eucalyptus oil or 1,8-cineole was applied to BMMCs sensitised with anti-DNP-IgE 24 h before stimulation with DNP-human serum albumin, and then after 10 min, cells were lysed, separated by SDS-PAGE and immunoblotted to analyse p-Syk, Syk, p-Lyn and Lyn expression. The left side presents representative examples of blots for each protein, and the right bar graphs present the phosphorylation relative ratios for Syk and Lyn.Phosphorylation ratios were calculated relative to Syk and Lyn expression (each set as 1), and data are presented as the mean ± SEM of 4–6 independent experiments. Dunnett’s test was used to assess the statistical significance (**P < 0.01 versus DNP-HSA). The original blots are shown in the supplementary Fig. 5.
