Figure 2
From: Single cell profiling of capillary blood enables out of clinic human immunity studies
Diurnal variability in subpopulations of capillary blood (a) Magnitude (Z-score) of the difference in AM vs PM gene expression across the whole population of cells (x) vs the cell type with the largest magnitude Z-score (y). Points above or below the significance lines (FDR < 0.05, multiple comparison correction) display different degrees of diurnality. The size of each marker indicates the abundance of the gene (the largest percent of cells in a subpopulation that express this gene). (b) Distribution of expression of DDIT4, a previously identified circadian rhythm gene22, shows diurnal signal across all cells, as well as individual cell types, such as natural killer (NK) cells. u indicates the mean fraction of transcripts per cell (gene abundance). (c) Example of newly identified diurnal genes, LSP1 and IFI16 that could be missed if analyzed at the population level (d) Example of a gene, EAF2, that could be falsely classified as diurnal (i) without considering cell type subpopulations due to a diurnal B cell abundance shift (ii).
