Figure 3
From: Minimally invasive system to reliably characterize ventricular electrophysiology from living donors
Viability evaluation by colorimetric measurement of enzyme activity. (A) Representative images of TTC staining of pig transmural tissue blocks and transmural core biopsies as well as human papillary muscles and transmural core biopsies. Non-viable transmural biopsies (right image) are shown for comparison. All tissues showed widely extended homogeneous deep red staining correlating with viable tissue. (B) Quantitative evaluation of viability for transmural core biopsies as relative to tissue blocks in pigs and relative to papillary muscles in humans, with no statistically significant differences in the absolute values in any of the two cases. Differences between relative viabilities in pig and human biopsies were not statistically significant. n.s. non-statistically significant differences according to Mann–Whitney U-test. (C) Effects of vibratome slicing on tissue viability by quantification of relative viability of the slices with respect to each corresponding tissue. Slicing led to significant differences in absolute absorbance values only for transmural core biopsies. Relative variabilities were statistically significantly different between transmural biopsy slices versus larger tissues for pigs but not for humans. *p < 0.05 and n.s. non-statistically significant differences according to Mann–Whitney U-test. n/N = 8/2 for pig tissue blocks, n/N = 104/6 for pig transmural biopsies, n/N = 4/3 for human papillary muscles and n/N = 77/7 for human transmural biopsies.
