Figure 3
The ZIKV-induced dsRNA-positive CER region is a dense tubular matrix. (A) Shown are representative mock-infected cells and CER (green) and PER (red) masks overlaid with the dsRNA-positive ER mask (white) for ZIKV-infected ERmoxGFP and Sec61β-GFP transfected U87 cells. Enlarged images of ERmoxGFP transfected cells show 3 × 3 μm ROIs of the mock-infected CER (green box) and of the ZIKV-infected dsRNA-positive (red box) and dsRNA-negative (yellow box) CER shown in (B). Graphs show the volume percent of the CER or PER region that contains dsRNA-positive ER (left) and the volume percent of the dsRNA-positive ER that resides within the CER mask. (B) 2D images of ER (white) and dsRNA (red) labeling in 3 × 3 μm ROIs of the ZIKV-infected dsRNA-positive (red) and dsRNA-negative (yellow) CER and mock-infected (green) CER are shown above Imaris 3D surface rendering of 1 × 1 μm regions of the above ROIs. Graph shows mean normalized ER density for each of the three CER zones by Imaris segmentation and masking. 5 cells per biological replicate were analyzed for a total of N = 3. ANOVA with post-hoc Tukey HSD: * = P < 0.05, ** = P < 0.01, and *** = P < 0.001. Error bars represent SEM. Scale bar: 10 microns (500 nm for zoomed ROIs).
