Figure 5
In vitro cardiovascular tissue formation in the multiscale scaffold. (a) (left) Immunofluorescence images for CD31 in the fibrin hydrogel region of the multiscale scaffold at each time point. Yellow dashed rectangle insets show × 40 magnified images of each group. Scale bars = 200 μm and 100 μm in low magnification and high magnification images, respectively. (right) Quantitative analysis of CD31 expression by qRT-PCR at 3-week culture. (b) Quantitative expression of cardiac markers (MHC, ACTC1) from ADSCs in the multiscale scaffold by qRT-PCR at 3-week culture. The expression levels to relative to the housekeeping gene (GAPDH) were calculated for analysis of qRT-PCR results (n = 3). Data are mean ± s.d. One-way ANOVA with Tukey’s post-hoc test was used. ***P < 0.001, **P < 0.01.
