Figure 1

lipogenesis of BAs partially contributes to postnatal iBAT growth. Interscapular brown adipose tissues (iBAT) were collected from mice at postnatal day 1, 4, 8, 12, 15 and 30. (A,B) Measurement of iBAT weight (A) and body weight (B) at different ages. (C) iBAT weight and body weight ratio. (D) Immunofluorescence staining images of iBAT tissue at different ages. MCT1 (monocarboxylate transporter 1) antibody was used to visualize plasma membrane of BAs. Bar = 50 µm. € Quantification of cross-sectional area of BAs. N = 4–6. (A, C and E) One-way ANOVA post-hoc Tukey's multiple comparisons test, *P < 0.05; ***P < 0.001. (F) Folds change comparison between iBAT weight and brown adipocyte volume. iBAT weight and brown adipocytes volume at different ages were normalized to the corresponding values of P1, respectively. Student t test, *P < 0.05; **P < 0.01. N = 4–6.