Figure 3
Analysis of BAs DNA synthesis activity. iBAT collected at indicated ages was used for analysis. (A) Representative immunofluorescence images of iBAT labeled with EdU. EdU was subcutaneously injected into mouse pups 24 h before iBAT collection. Bar = 50 µm. (B) Quantification of EdU positive BAs. N = 4 for each time points. (C–E) qRT-PCR measurement of gene expression at different ages. Total RNA from iBAT was used for qRT-PCR. (B–E) One-way ANOVA with post-hoc Tukey's multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001. N = 4.
