Figure 5 | Scientific Reports

Figure 5

From: Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice

Figure 5

Evaluation of BAs nuclearity and DNA ploidy in juvenile mice. (A) Representative images of dissociated BAs. Bar = 50 µm. (B) Measurement of BAs nuclearity at P1, P8, P15 and P30. Dissociated BAs were stained with DAPI and counted for nucleus number. Data were collected from three independent assays. sNuc: single nucleus. BiNuc: binucleus. (C) TdTom + BAs gating image. (D) DNA content histogram of gated tdTom + BAs. Population 2 (P2) and Population 3 (P3) represent diploid and tetraploid BAs, respectively. The cell number of P2 and P3 was normalized to the number of gated tdTom + BAs (P1), and calculated percentages were shown in the inset bar graph. N = 4. (E) Schematic summary of the current findings. In the first two weeks, BAT expands by increasing BAs number (proliferation) and size (lipogenesis); from P15 to P30, lipogenesis but not proliferation of the BAs contributes to BAT expansion.

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