Figure 2
Expression of COL6A3 and PRRX1 in mature adipocytes and stromal vascular fraction (SVF). Adipocytes and SVF from human subcutaneous and omental whole tissue biopsies were separated by collagenase treatment, and gene expression was measured by qPCR using TBP as a reference gene. (A) The expression of PRRX1 was measured in subcutaneous adipocytes and SVF from morbidly obese people (n = 13, SVF n = 12), subcutaneous adipocytes in non-obese subjects (adipocyte n = 17, SVF n = 10), and omental adipocytes in severely obese subjects (adipocyte n = 13, SVF n = 12). Data are presented as mean ± SEM. (B) PRRX1 and COL6A3 mRNA were correlated in mature subcutaneous adipocytes and SVF (log2-transformed data, Pearson correlation). SC, subcutaneous; SVF, stromal vascular fraction. **p value < 0.01 (relative to obese SC for adipocytes); §§p value < 0.01 (relative to obese SC for SVF).
