Figure 5 | Scientific Reports

Figure 5

From: Antiseptic quaternary ammonium compound tolerance by gram-negative bacteria can be rapidly detected using an impermeant fluorescent dye-based assay

Figure 5

RFDMIA of various Gram-negative species to determine QAC susceptibility. The results of stationary phase RFDMIA of E. coli (EC) (A, B), A. baumannii (AB) (A, B), P. aeruginosa (PA) (A, B), K. pneumoniae (KP; KPBZKT) (C), and S. sonnei (SS; SSBZKT) (D) exposed to increasing concentrations of QAC are shown. For each RFDMIA plot shown, a Student’s two-tailed t test was calculated by comparing every QAC concentration PI ΔRFUΔ30min value to the lowest measured QAC concentration PI ΔRFUΔ30min value shown on the plot. This t test was used to identify the lowest QAC concentration with a significant increase in PI ΔRFUΔ30min value, which are indicated as a double asterisk (**) with P < 0.01. The horizontal lines with asterisks indicate interspecies PI ΔRFUΔ30min value comparisons at the same QAC concentrations. Data represents three bacterial bioreplicates (n = 3) measured from averaged technical triplicate measurements.

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