Table 1 A summary of mean BZK and CET MIC and 30MBC values of all bacterial isolates tested in this study based on a twofold (log₂) dilution series from three bacterial bioreplicates measured in technical triplicate.

Bacterial isolate tested in this study; isolate abbreviation	MIC (μg/mL) (n = 9)*		30MBC (μg/mL) (n = 9)*
	BZK	CET	BZK	CET
Escherichia coli K-12 BW25113; EC^a	18.8	37.5	75.0	75.0
Escherichia coli K-12 BW25113; ECBZKT^b	150.0	150.0	150.0	150.0
Escherichia coli K-12 BW25113; ECCETT^c	75.0	300.0	150.0	300.0
Acinetobacter baumannii DSM 6974; AB^d	18.8	37.5	75.0	75.0
Pseudomonas aeruginosa PA01 DSM 22,644; PA^d	75.0	150.0	150.0	300.0
Klebsiella pneumoniae DSM 6135; KP^d	18.8	–	37.5	–
Klebsiella pneumoniae DSM 6135; KPBZKT^e	75.0	–	150.0	–
Shigella sonnei DSM 5570; SS^d	9.4	–	37.5	–
Shigella sonnei DSM 5570; SSBZKT^f	37.5	–	75.0	–

*n = 9 refers to 3 biological replicate cultures and 3 technical replicate dilutions used for AST.
^aIsolate sourced from the Yale Coli Genetic Stock Centre; CGSC (https://cgsc.biology.yale.edu/).
^bBZK-adapted BW25113 isolate, tolerant to 75 µg/mL BZK (BZKR). Refer to reference BioRXIV# 201,814.
^cCET-adapted BW25113 isolate, tolerant to 150 µg/mL CET (CETR). Refer to reference BioRXIV# 201,814.
^dStrain sourced from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH; DSMZ (https://www.dsmz.de/).
^eBZK-adapted DSM 6135 isolate, tolerant to 75 µg/mL BZK.
^fBZK-adapted DSM 5570 isolate, tolerant to 37.5 µg/mL BZK.

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