Table 1 Designed primers of quantitative real-time PCR.
Gene name | Forward primer sequence | Reverse primer sequence | Product size/bp | Annealing temperature/°C |
|---|---|---|---|---|
AcSPS1 | CTATCAATGACAAGAAGGGCGAAAA | GCAACGGTGAGCCTGAATCCT | 158 | 63.6 |
AcSPS2 | ATGCTTTTCACTGGTCACTCACT | CATCATACAGACGCCATTGCTC | 199 | 58.2 |
AcSPS3 | TTCTGAAGTTGGTCCTTTTGGG | TCGTCTTCAGCAACTTGTCCTC | 165 | 61.2 |
AcSPS4 | ACGAGCACCAAGCAGGAGATT | CCACAACATTGCTGAAGTCCATA | 169 | 61.3 |
AcSPS5 | GGAGAAGGAAAAGGGTGATGC | CCTGACCTCCAGTGTCTGAATC | 191 | 58.4 |
AcSUS1 | AACTTGGGATTACTCTGGGAACT | ATTTCTTGGTATGTGCTGGTGAT | 175 | 58.5 |
AcSUS2 | GGTGGCTTACAGAAGGCTCAG | CCACTGCCTAAACCTTTGCTC | 195 | 59.2 |
AcSUS3 | GTTGCTTTGGCAGTGAGGAGG | GGTTAGGCGACGACTTGAGAAA | 198 | 61.5 |
AcSUS4 | TCAAAGAATACAACTTGGATGGC | CAAGTGGCAAATGTTGGAAGC | 187 | 59.4 |
AcSUS5 | TTGGGCTATCCTGACACTGGT | CAATGAGAATGCGTGGAATGA | 124 | 59.4 |
AcSUS6 | ACACTGGTGGTCAGGTGGTTT | GCGAATGTTCTGCTCCGTAAA | 189 | 59.8 |
AcSUS7 | ATCAAAGCCCATTATCTTCTCCA | TGACTTCTTCACATCATTGTAACCC | 145 | 60.1 |
