Figure 4

Cellular Development, Cellular Growth and Proliferation, Embryonic Development network in comparison of n-3 PUFA, pregnant, low diet versus n-3 PUFA, pregnant, high diet. The network is displayed graphically as nodes (genes). The node colour indicates the expression of genes with red representing up-regulation and green indicating lower expression in the n-3 PUFA supplemented pregnant heifers on the high plane of nutrition post-insemination, compared to the n-3 PUFA pregnant heifers on the low plane of nutrition. The network displays nodes (genes/gene products) and edges (the biological relationship between nodes). The colour intensity of the nodes indicates the fold-change increase (red) or decrease (green) associated with a particular gene in control animals compared to n-3 PUFA supplemented. A solid line indicates a direct interaction between nodes (genes/gene products), and a dashed line indicates an indirect relationship between nodes. The shape of the node is indicative of its function. The network image was generated through the use of IPA (QIAGEN Inc., https://www.qiagenbio-informatics.com/products/ingenuity-pathway-analysis)²².