Figure 2
From: TRPM2 promotes autophagic degradation in vascular smooth muscle cells
Knockout of TRPM2 reduced starvation-induced change in autophagosomes and autolysosomes. Shown were representative fluorescent images (A, B) and data summary (C, D) demonstrating the effect of TRPM2 KO on starvation-induced change in autophagosomes (yellow dots in merged images) and autolysosomes (red alone) in mASMCs. The cells were transfected with adenovirus containing mCherry-GFP-LC3 tandem reporter. 3 h starvation reduced the number of autophagosomes but increased the number of autolysosomes. Knockout of TRPM2 inhibited the starvation-induced reduction of autophagosomes. Values are mean ± SEM (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001; ns = not significant). One-way ANOVA was used for statistical test.
