Figure 5

Immunostaining for glial fibrillary acidic protein (GFAP) in retinas of a rat treated with intravitreal oligomeric Aβ42. Retinal sections from the peripheral retina in the experimental eyes (a, c) show significant GFAP staining of cells with a typical morphology for Müller cells. (b, d) Peripheral retinal areas from the control eyes demonstrate no labelling of Müller cells. (Red- GFAP, Blue- DAPI staining of all nuclei). To obtain this data 16-μm tissue sections at five different tissue planes (100 μm apart) were prepared and stained. Results were replicated in four rats in each group. Scale bars: 50 µm.