Figure 2 | Scientific Reports

Figure 2

From: Transcriptomic analysis of tobacco-flavored E-cigarette and menthol-flavored E-cigarette exposure in the human middle ear

Figure 2

E-liquids stimulated the expression of inflammatory cytokines in HMEEC-1. Cells were treated with tobacco-flavored e-liquid and menthol-flavored e-liquid at the IC50 concentration for 24 h. Quantitative real-time PCR was performed to evaluate inflammatory cytokine gene expression levels. The expression levels of COX-2 and TNF-α increased when cells were exposed to the e-liquids, and the mRNA expression of cytokines was significantly elevated in the tobacco-flavored e-liquid group (p < 0.05).

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