Figure 7

Responses of AnxA5-deficient or AnxA6-deficient MDA-MB-231 cells to a membrane damage by laser ablation. Sequence of representative images showing the response of an AnxA5-deficient (a) or an AnxA6-deficient MDA-MB-231 (b) cell after membrane damage performed by laser irradiation in the presence of FM1-43 (green) is presented. Cells were treated and presented as described in the legend of Fig. 4. Scale bar: 10 μm. (c) Kinetic data represent the FM1–43 fluorescence intensity integrated over whole cell sections, averaged for about 60 cells (±SD) from five independent experiments (see also Supplementary table 2). The response of control (control MDA), AnxA5-deficient (MDA kd AnxA5) and AnxA6-deficient (MDA kd AnxA6) MDA-MB-231 cells are presented. Control cells were MDA-MB-231 cells transduced with SCshRNA-containing lentiviral particles. For MDA kd AnxA5 and MDA kd AnxA6 a continuous and large increase of the fluorescence intensity was observed, indicating the absence of membrane resealing. (d) Zoomed and unsaturated images of kd AnxA5 and kd AnxA6 cells presented in a and b 120 s after membrane injury, respectively. Large filled and small dashed white arrows point out the wound site and the lipid material accumulating inside the cell after membrane injury, respectively. Scale bar: 10 μm.