Figure 8

Responses of Anx-deficient MDA-MB-231 cells to membrane damages induced by migration on collagen I. 2·10⁵ control, AnxA5-deficient, AnxA6-deficient or AnxA5-AnxA6 deficient MDA-MB-231 cells were seeded on a glass coverslip coated with collagen I. Control cells were MDA-MB-231 cells transduced with SCshRNA-containing lentiviral particles. 24 h (left panels) and 48 h (middle panels) after seeding, cells were imaged by phase contrast microscopy. At 48 h, the insert displays a magnified area from the image for the sake of clarity. At 48 h-post seeding, the percentage of cells displaying rounded shape was estimated using the ImageJ software. Rounded cells were defined as presenting a circularity ≥ 0.50, with circularity = 4*pi*(area/perimeter^2). Cells were then washed, trypsinized and counted. Mean values (±SD) from three independent experiments performed in triplicate are presented in the right lane. Student t Test for independent samples. **p < 0.01. Scale bars: images, 100 μm; inserts, 25 µm.