Figure 6
From: SHROOM3, the gene associated with chronic kidney disease, affects the podocyte structure
Shroom3 knockdown decreased podocyte lamellipodia. Rat glomerular epithelial cells transfected with either Stealth RNAi Negative Control Med GC (Invitrogen, Carlsbad, CA) or with predesigned Shroom3 siRNA were used. Western blotting and immunofluorescence were performed at 48Â h after knockdown of Shroom3. (a) By Western blotting, the reduced level of Shroom3 was confirmed in si-Shroom3 treated cells. (b) Representative images of cell shape and phalloidin in scramble siRNA- and si-Shroom3-treated cells. Scramble siRNA-treated cells are larger and lamellipodia were well formed while si-Shroom3-treated cells are small and less lamellipodia were formed. (c) Quantitative analysis of cell area and lamellipodia formation. The result is consistent with (b) representative images.
