Figure 3

Contribution of higher aggregation propensities of 4 examined TFs to [SWI⁺] DEGs. (a–c) The 4 TFs are more aggregable in [SWI⁺] cells than in non-prion ([swi⁻]) cells among 30 tested TFs that were predicted having a higher aggregation propensity and with overrepresented targets in [SWI⁺]-DEGs (see Table S2 for details). To avoid unnecessary overproduction, 0.02% galactose was used to induce the expression of the indicated proteins from a single-copy plasmid. Representative images of the indicated YFP fusions are shown in panel (a), and quantitative results are shown in panel (b) (Swi1-NQ-YFP as positive control and YFP as negative control) and in (c) (for tested TFs). (d) Reduced activity of the 4 TFs due to aggregation explains the inhibition (upper) and activation (bottom) of [SWI⁺] DEG targets when they function as activators and inhibitors, respectively. All TFs in the regulatory network are highlighted by blue.