Figure 6

Analyses of protein synthesis and Environmental Stress Response (ESR) of swi1∆ and [SWI⁺] cells. (a) KEGG ribosome gene enrichment analysis was performed as described in the Methods. ↑ and ↓ indicate upregulation and downregulation by swi1∆ or [SWI⁺], respectively. (b) Left: shown are the steady state protein levels of the indicated strains. The total protein concentration normalized to cell number was determined for log-phase cells by a protein assay as described in the method. Right: images showing cell size and growth phenotype of the three strains used in this study. (c) Shown are fractions of environmental stress response (ESR) genes including iESR and rESR genes that are either activated or repressed in [SWI⁺] and swi1∆ cells. The significance was analyzed by Fisher’s exact test for panels of (a,c) and Student T-test for panel (b). *p-value < 0.05; **p-value < 0.001; ***p-value < 0.001. (d,e) Shown are cell viability assays of the three tested strains upon the indicated treatments as described in the “Methods”.