Figure 1
Iron overload decreases the protein expression level of several signaling molecules and the cell viability in RPH. (A) RPH were treated with the indicated concentration of FeCl3 for 24 h. Expression level of AMPKα, SMAD1, ERK, JNK, LC-3B, and β-actin was evaluated by Western blot analysis. (B) RPH were treated with the indicated concentration of FeCl3 in the presence or absence of FBS for 24 h, and thereafter the cell viability was measured. The cell viability of untreated control cells was set at 100. * and †: significantly decrease (P < 0.05), as compared with cells treated without FeCl3 in the absence of FBS and presence of FBS, respectively.
