Figure 4
Hepatic cell lines do not undergo cell death and also do not show a decrease in AMPK and LC-3B protein levels in response to FeCl3 treatment. (A) HepG2 cells and Hepa1-6 cells were treated with the indicated concentration of FeCl3 in the absence of FBS for 24 h, and thereafter the cell viability was measured. Cell viability of untreated cells was set at 100. (B) RPH, HepG2 cells, and Hepa1-6 cells were treated with different concentration of FeCl3 (0, 12.5, 25, 50, 100, 200, 400, or 800 μM) for 24 h. Expression of AMPKα, LC-3B, and β-actin was evaluated using Western blot analysis.
